验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84133-5-RR targets CFAP70 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | CFAP70 fusion protein Ag35209 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tetratricopeptide repeat domain 18 |
| 别名 | 241177C9, TPR repeat protein 18, TTC18 |
| 计算分子量 | 126 kDa |
| 观测分子量 | 110 kDa, 128 kDa |
| GenBank蛋白编号 | BC032856 |
| 基因名称 | TTC18 |
| Gene ID (NCBI) | 118491 |
| RRID | AB_3671691 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q5T0N1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cilia- and flagella-associated protein 70 (CFAP70), also known as TTC18, harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CFAP70 antibody 84133-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |