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验证数据展示

  • WB analysis using 27688-1-AP

    WB analysis using 27688-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27688-1-AP (TP73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 27688-1-AP

    IHC staining of human placenta using 27688-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27688-1-AP (TP73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 27688-1-AP

    IHC staining of human skin cancer using 27688-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 27688-1-AP (TP73 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TP73 Polyclonal antibody

TP73 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27688-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

P73, tumor protein p73

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue
Positive IHC detected inhuman skin cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27688-1-AP targets TP73 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag26686

Product name: Recombinant human TP73 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001126240

Sequence: MAQSTATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLEGMTTSVMAQFNLLSSTMDQMSSRAASASPYTPEHAASVPTHSPYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein p73
别名 P73, tumor protein p73
计算分子量 70 kDa
观测分子量70 kDa
GenBank蛋白编号NM_001126240
基因名称 TP73
Gene ID (NCBI) 7161
RRIDAB_3669616
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15350
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP73 is a member of the TP53 family whose expression has been observed altered in most human cancers and associated with the prognosis. TP73 translates into a complex number of isoforms with both oncogenic and tumor-suppressor functions and presents a complex cross-talk with other members of the family (TP53 and TP63).

实验方案

Product Specific Protocols
WB protocol for TP73 antibody 27688-1-APDownload protocol
IHC protocol for TP73 antibody 27688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols