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验证数据展示

  • IF Staining of HEK-293 using 67574-1-Ig

    IF Staining of HEK-293 using 67574-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67574-1-Ig

    WB analysis using 67574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67574-1-Ig (TBC1D25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of NIH/3T3 using 67574-1-Ig

    IF Staining of NIH/3T3 using 67574-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TBC1D25 antibody (67574-1-Ig, Clone: 2C7H3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

TBC1D25 Monoclonal antibody

TBC1D25 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67574-1-Ig

产品说明书

CloneNo. 2C7H3
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (2)

别名

2C7H3, TBC1 domain family member 25, TBC1 domain family, member 25

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, Neuro-2a cells
Positive IF/ICC detected inNIH/3T3 cells, HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67574-1-Ig targets TBC1D25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag29470

Product name: Recombinant human TBC1D25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 32-299 aa of BC101817

Sequence: EREVVRVRVKKCESFLPPEFRSFAVDPQITSLDVLQHILIRAFDLSGKKNFGISYLGRDRLGQEVYLSLLSDWDLSTAFATASKPYLQLRVDIRPSEDSPLLEDWDIISPKDVIGSDVLLAEKRSSLTTAALPFTQSILTQVGRTLSKVQQVLSWSYGEDVKPFKPPLSDAEFHTYLNHEGQLSRPEELRLRIYHGGVEPSLRKVVWRYLLNVYPDGLTGRERMDYMKRKSREYEQLKSEWAQRANPEDLEFIRSTVLKDVLRTDRAH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TBC1 domain family, member 25
别名 2C7H3, TBC1 domain family member 25, TBC1 domain family, member 25
观测分子量 80 kDa
GenBank蛋白编号BC101817
基因名称 TBC1D25
Gene ID (NCBI) 4943
RRIDAB_2882787
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ3MII6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TBC1D25 antibody 67574-1-IgDownload protocol
WB protocol for TBC1D25 antibody 67574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC,IF

In Vitro Cell Dev Biol Anim

TBC1 domain family member 25 protects against myocardial apoptosis and the proinflammatory response triggered by ischemia-reperfusion injury through suppression of the TAK1-JNK/p38 MAPK signaling cascade

Authors - Ziwen Liu
View Article
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