ChromoTek Spot VHH, recombinant binding protein

Alpaca anti-Spot VHH, purified recombinant binding protein

Specificity

Spot-Tag®

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Cat No : etb

Synonyms

Spot Nanobody, and Spot-Label (unconjugated protein)



Product Information

Alpaca anti-Spot VHH, purified recombinant binding protein

DescriptionAlpaca anti-Spot VHH, purified recombinant binding protein binds extraordinary stable & reliable to Spot-Tag®:

• Stable up to 65 °C, 10 mM DTT, 0.75 M Guanidinium•HCl, 2 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.1 % SDS, 1 % Triton X-100, 1 % Dexoycholate

• pH 4-10

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsSuitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Spot VHH contains a sortase-tag and a His-tag at its C terminus: LPETGHHHHHH. This sequence includes the sortase recognition motif LPETG, for conjugation. In addition, bivalent Spot-VHH may be detected by an anti-His antibody. The Spot VHH corresponds to the “BC2-Nanobody” used by Virant et al in the publication "Nature Communications" (2018) doi:10.1038/s41467-018-03191-2 refdoi:10.1038/s41467-018-03191-2
Specificity/TargetSpot-Tag® sequence PDRVRAVSHWSS
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Molecular Weight30.3 kDa
RRIDAB_2827572
Storage Buffer25 mM TAPS pH8,5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.

Publications

ApplicationTitle
IF

Sci Adv

Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.

Authors - Filipe Nunes Vicente
WB

iScience

ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodes.

Authors - Jessica M Hong

Cells

Cep192, a Novel Missing Link between the Centrosomal Core and Corona in Dictyostelium Amoebae.

Authors - Valentin Pitzen
WB

Cells

The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.

Authors - Mohamed Ibrahem Elhawy

Life Sci Alliance

The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.

Authors - Kenji Shimada