ChromoTek Spot-Cap™ and Peptide

Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.

Specificity

Spot-Tag®

Applications

Protein purification

Conjugate

Agarose

Cat No : eca-ep

Synonyms

Spot Affinity Gel; Spot Affinity Matrix



Product Information

Spot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins.

DescriptionSpot-Cap® is a Nanobody-based affinity resin optimized for one-step protein purification of Spot-tagged proteins:

• Economic: high binding capacity and multiple regeneration cycles

• Fast and gentle one-step purification at 4°C preserves protein stability and function

• Tight and highly selective binding of a single repeat Spot-Tag

• High specificity leads to minimal contamination of host cell proteins

• No contamination of antibody heavy and light chains

ApplicationsProtein purification
Specificity/TargetSpot-Tag® PDRVRAVSHWSS
Binding capacity10 mg protein (30 kDa) / 1 mL settled resin
(340 nmol / 1 mL settled resin)
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution buffer0.1 mM Spot-peptide or 100 mM glycine pH 2.0 at 4°C
Wash buffer compatibility1 M NaCl, 2 M Urea, 10 mM DTT, 10 mM ß-mercaptoethanol, 10 mM TCEP, 2% DDM, 2% NP-40, 2% Triton X-100
pH range for purificationpH 4 - 11
Regeneration buffer100 mM glycine pH 2.0
Regeneration>5 cycles
Purification format Batch and gravity flow
Affinity (KD) Dissociation constant KD of 40 nM
RRIDAB_2827549
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature.
Upon receipt store at 4°C. Do not freeze!
Stable for one year.