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SpeedAb WB Kit for Mouse IgG2b

Reactivity:

Mouse IgG2b

Application:

WB

Conjugate:

HRP

Cat no : SW004

Print datasheet

Validation Data Gallery

  • SpeedAb WB Kit for detection of mouse IgG2b. ​ (red) Classical Western blot detection of GAPDH in lysates from different human cell lines. A primary anti-GAPDH antibody (mouse IgG2b) was used in combination with a secondary anti-mouse antibody (HRP), after blocking and incubation with antibodies for 1 h, respectively. The total time of ~ 5 h includes SDS-PAGE, blotting, blocking, primary and secondary antibody incubation, and washing steps of the membrane.​ (blue) Detection of GAPDH in lysates from different human cell lines via SpeedAb WB Kit. The same primary anti-GAPDH antibody was labeled with FlexAble HRP (anti-mouse IgG2b) via a simple 10 min protocol, enabling a 1-step detection. The blotting accelerator speeds up binding of the antibody to the target protein, cutting the antibody incubation time to 15 min only and the total time including SDS-PAGE, blotting, antibody incubation and a final washing step to ~2 h.​

    SpeedAb WB Kit for detection of mouse IgG2b. ​ (red) Classical Western blot detection of GAPDH in lysates from different human cell lines. A primary anti-GAPDH antibody (mouse IgG2b) was used in combination with a secondary anti-mouse antibody (HRP), after blocking and incubation with antibodies for 1 h, respectively. The total time of ~ 5 h includes SDS-PAGE, blotting, blocking, primary and secondary antibody incubation, and washing steps of the membrane.​ (blue) Detection of GAPDH in lysates from different human cell lines via SpeedAb WB Kit. The same primary anti-GAPDH antibody was labeled with FlexAble HRP (anti-mouse IgG2b) via a simple 10 min protocol, enabling a 1-step detection. The blotting accelerator speeds up binding of the antibody to the target protein, cutting the antibody incubation time to 15 min only and the total time including SDS-PAGE, blotting, antibody incubation and a final washing step to ~2 h.​

Product Information

SpeedAb Western Blot Kits are ideal for fast western blot workflows, cutting down protocol time of your experiment to ~2h, while also minimizing hands-on time.

The Kit combines two Proteintech products:

FlexAble HRP enables single step detection by fast & easy labeling of your primary antibody with an HRP-conjugated FlexLinker. FlexAble labeling can be done in under 20 min, requires no additional material, works with any Rabbit IgG (Proteintech #SW001), Mouse IgG1 (#SW002), Mouse IgG2a (#SW003), or Mouse IgG2b (#SW004) antibody from any supplier, in almost every buffer system, at any concentration.

Learn more about FlexAble: https://www.ptglab.com/products/flexable-antibody-labeling-kits/

Blotting Accelerator reduces incubation times per antibody to ~15min without sacrificing result quality.

Learn more about Blotting Accelerator: https://www.ptglab.com/products/-TM-WB--PR20039.htm

When combined, these two components facilitate easy, and reliable 1 step detection using rabbit antibodies in 15 minutes.

Product Codes for specific Kits:

(FlexLinkers are species specific and will only work with the correct IgG subtype)

(SW001) Rabbit IgG

(SW002) Mouse IgG1

(SW003) Mouse IgG2a

(SW004) Mouse IgG2b

产品成分

Component

CatalogNo.

Size

FlexAble HRP Antibody Labeling Kit for Mouse IgG2b

KFA065

10 rxns

Blotting Accelerator

PR20039

100 mL

保存条件

FlexAble HRP Kits: Store for 1 year at -20°C or for 6 months at 4°C upon receipt. Avoid exposure to light.

Blotting Accelerator: Store at 2-8℃ for a year.

使用方法

Sizing is based on a standardized antibody incubation volume of 5 mL Blotting Accelerator (single step detection) and a recommended dilution of 1:5000, with 0.5 ug of antibody used per Blot.

Example:

Recommended dilution of primary antibody: 1:5000

Volume of Accelerator per reaction: 5000 uL

Volume of antibody needed: 1 uL

Antibody concentration: 0.5 ug/uL

Amount of antibody to conjugate: 0.5 ug

Amount of FlexAble reactions needed: 1 rxn

Scale volumes & amounts according to recommended dilution of your primary antibody.

We recommend using a minimum of 1 uL FlexLinker, even if the antibody to be labeled is less than 0.5 ug (e.g. in cases when recommended dilution for WB is very high).

If you need help calculating your exact volumes, use our FlexAble Calculator Tool: https://www.ptglab.com/FlexCalc

Documentation

Datasheet
SpeedAb WB Kit for Mouse IgG2b Datasheet