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Sam68 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse, rat

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat no : MP03453-2

Synonyms

KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03453-2, Human Sam68 Recombinant Matched Antibody Pair - PBS only. 88301-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0235. 88301-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

    Sandwich ELISA standard curve of MP03453-2

    Sandwich ELISA standard curve of MP03453-2, Human Sam68 Recombinant Matched Antibody Pair - PBS only. 88301-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0235. 88301-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

  • The mean Sam68 concentration was determined to be 17.46 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load, 6.52 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load and 683.88 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP03453-2

    The mean Sam68 concentration was determined to be 17.46 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load, 6.52 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load and 683.88 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP03453-2 targets Sam68 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: Sam68 Recombinant monoclonal antibody, PBS Only (Capture/Detector) 88301-1-PBS (260478D3). 100 μg. Concentration 1 mg/ml.

Detection antibody: Sam68 Recombinant monoclonal antibody, PBS Only (Detector) 88301-3-PBS (260478D5). 100 μg. Concentration 1 mg/ml.

Alternative Sam68 matched antibody pairs: MP03453-1

Matched pair protein standard: Ag0235

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 88301-1-PBS
Tested Reactivity human, mouse, rat
Immunogen Sam68 fusion protein Ag0235
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KH domain containing, RNA binding, signal transduction associated 1
Synonyms KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62
GenBank accession numberBC000717
Gene symbol Sam68
UniprotQ07666
Gene ID (NCBI) 10657
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 88301-3-PBS
Tested Reactivity human
Immunogen Sam68 fusion protein Ag0235
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name KH domain containing, RNA binding, signal transduction associated 1
Synonyms
GenBank accession numberBC000717
Gene symbol Sam68
UniprotQ07666
Gene ID (NCBI) 10657
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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