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验证数据展示

  • IF Staining of mouse brain using CL488-68522

    IF Staining of mouse brain using CL488-68522

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 STK24 antibody (CL488-68522, Clone: 2B4C1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated STK24 Monoclonal antibody

STK24 Monoclonal Antibody for IF-P
Cat No. CL488-68522

产品说明书

CloneNo. 2B4C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P

别名

2B4C1, EC:2.7.11.1, Mammalian STE20-like protein kinase 3, MST3, Serine/threonine-protein kinase 24 12 kDa subunit

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy® STK24 Ready-To-Use IHC Kit. STK24 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68522-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68522 targets STK24 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31526

Product name: Recombinant human STK24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 320-443 aa of NM_003576

Sequence: SDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase 24 (STE20 homolog, yeast)
别名 2B4C1, EC:2.7.11.1, Mammalian STE20-like protein kinase 3, MST3, Serine/threonine-protein kinase 24 12 kDa subunit
计算分子量49KD
观测分子量50 kDa
GenBank蛋白编号NM_003576
基因名称 STK24
Gene ID (NCBI) 8428
RRIDAB_3673023
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6E0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 STK24 antibody CL488-68522Download protocol
Standard Protocols
Click here to view our Standard Protocols
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