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验证数据展示

  • WB analysis using 68522-1-Ig

    WB analysis using 68522-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68522-1-Ig (STK24 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 68522-1-Ig

    IF Staining of rat brain using 68522-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using STK24 antibody (68522-1-Ig, Clone: 2B4C1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STK24 Monoclonal antibody

STK24 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68522-1-Ig

产品说明书

CloneNo. 2B4C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

Serine/threonine-protein kinase 24 36 kDa subunit, Serine/threonine-protein kinase 24 12 kDa subunit, MST3, Mammalian STE20-like protein kinase 3, EC:2.7.11.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF-P detected inrat brain tissue
Planning an IHC experiment? We recommend our IHCeasy® STK24 Ready-To-Use IHC Kit. STK24 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68522-1-Ig targets STK24 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31526

Product name: Recombinant human STK24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 320-443 aa of NM_003576

Sequence: SDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine/threonine kinase 24 (STE20 homolog, yeast)
别名 Serine/threonine-protein kinase 24 36 kDa subunit, Serine/threonine-protein kinase 24 12 kDa subunit, MST3, Mammalian STE20-like protein kinase 3, EC:2.7.11.1
计算分子量49KD
观测分子量50 kDa
GenBank蛋白编号NM_003576
基因名称 STK24
Gene ID (NCBI) 8428
RRIDAB_3085231
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y6E0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 24 (STK24), also named MST3, belonging to GCK subfamily of STE20-like kinases, acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation (PubMed: 10644707, PMID:12107159). STK24 and STK25 operate in the same cardiovascular development pathway with programmed cell death 10 (CCM3) (PMID: 20332113). It regulates axon outgrowth in forebrain neurons (PubMed: 17114295).

实验方案

Product Specific Protocols
IF protocol for STK24 antibody 68522-1-IgDownload protocol
WB protocol for STK24 antibody 68522-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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