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STAT3 phospho Tyr705 antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human

Applications

WB, Dot Blot

Cat No : 39595,39596 39595

Synonyms

STAT 3, STAT-3, APRF, HIES, FLJ20882, MGC16063, STAT3, pAb, polyclonal, STAT3 P-Y705, antibody, antibodies, sample, dbdabs

验证数据展示

  • STAT3 phospho Tyr705 pAb tested by Western blot. Hep G2 nuclear extract (15 ug per lane) was probed with STAT3 phospho Tyr705 polyclonal antibody (1:2,000). Lane 1: No treatment. Lane 2: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37°C).

    STAT3 phospho Tyr705 pAb tested by Western blot. Hep G2 nuclear extract (15 ug per lane) was probed with STAT3 phospho Tyr705 polyclonal antibody (1:2,000). Lane 1: No treatment. Lane 2: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37°C).

  • STAT3 phospho Tyr705 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT3 phospho Tyr705 pAb for STAT3 phospho Tyr705. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

    STAT3 phospho Tyr705 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT3 phospho Tyr705 pAb for STAT3 phospho Tyr705. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

产品信息

Tested Applications WB, Dot Blot

Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
ModificationPhosphorylated
Immunogen This STAT3 phospho Tyr705 antibody was raised against a peptide containing phosphorylated Tyr705 of human STAT3.
Full Name STAT3 phospho Tyr705 antibody (pAb)
Synonyms STAT 3, STAT-3, APRF, HIES, FLJ20882, MGC16063, STAT3, pAb, polyclonal, STAT3 P-Y705, antibody, antibodies, sample, dbdabs
Molecular weight 92 kDa
GenBank accession numberNP_003141
RRIDAB_2793272
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

STAT3 (signal transducer and activator of transcription 3) is a member of the STAT family of transcription factors. STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.