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验证数据展示

  • IHC staining of mouse embryo using 16725-1-AP

    IHC staining of mouse embryo using 16725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 16725-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOX15 antibody included
  • IF Staining of mouse embryo using 16725-1-AP

    IF Staining of mouse embryo using 16725-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 16725-1-AP (SOX15 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 16725-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

IHC, IF-P, ELISA and More (1)

别名

Transcription factor SOX-15, SRY-box transcription factor 15, SOX-15, SOX 15, Protein SOX-20

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse embryo tissue
Planning an IHC experiment? We recommend our IHCeasy SOX15 Ready-To-Use IHC Kit. SOX15 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

16725-1-AP targets SOX15 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human, pig
免疫原

CatNo: Ag10173

Product name: Recombinant human SOX15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-233 aa of BC000985

Sequence: MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQNPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGNLASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHYLPPGSPTPYNPPLAGAPMPLTHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 15
别名 Transcription factor SOX-15, SRY-box transcription factor 15, SOX-15, SOX 15, Protein SOX-20
计算分子量 25 kDa
GenBank蛋白编号BC000985
基因名称 SOX15
Gene ID (NCBI) 6665
RRIDAB_2878305
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60248
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SOX15 antibody 16725-1-APDownload protocol
IHC protocol for SOX15 antibody 16725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,pigIF

Cell Rep

Integration of single-cell transcriptome and chromatin accessibility of early gonads development among goats, pigs, macaques, and humans

Authors - Min Chen
View Article
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