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NHE8 Polyclonal antibody

NHE8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18318-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, mouse colon tissue, mouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human colon tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18318-1-AP targets NHE8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NHE8 fusion protein Ag13111 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 8
别名 KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8
计算分子量 581 aa, 65 kDa
观测分子量 70-85 kDa
GenBank蛋白编号BC112213
基因名称 SLC9A8
Gene ID (NCBI) 23315
RRIDAB_2270442
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2E8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.

实验方案

Product Specific Protocols
WB protocol for NHE8 antibody 18318-1-APDownload protocol
IHC protocol for NHE8 antibody 18318-1-APDownload protocol
IF protocol for NHE8 antibody 18318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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