验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse testis tissue, mouse colon tissue, mouse liver tissue, mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human colon tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
18318-1-AP targets NHE8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag13111 Product name: Recombinant human NHE8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 471-581 aa of BC112213 Sequence: IRLMDIEDAKAHRRNKKDVNLSKTEKMGNTVESEHLSELTEEEYEAHYIRRQDLKGFVWLDAKYLNPFFTRRLTQEDLHHGRIQMKTLTNKWYEEVRQGPSGSEDDEQELL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 9 (sodium/hydrogen exchanger), member 8 |
| 别名 | KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8 |
| 计算分子量 | 581 aa, 65 kDa |
| 观测分子量 | 70-85 kDa |
| GenBank蛋白编号 | BC112213 |
| 基因名称 | SLC9A8 |
| Gene ID (NCBI) | 23315 |
| RRID | AB_2270442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2E8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NHE8 antibody 18318-1-AP | Download protocol |
| IHC protocol for NHE8 antibody 18318-1-AP | Download protocol |
| IF protocol for NHE8 antibody 18318-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
