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验证数据展示

  • WB analysis using 30031-1-AP

    WB analysis using 30031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 30031-1-AP

    IHC staining of human placenta using 30031-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC43A2 antibody included
  • IHC staining of human placenta using 30031-1-AP

    IHC staining of human placenta using 30031-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLC43A2 antibody included
  • FC experiment of Jurkat using 30031-1-AP

    FC experiment of Jurkat using 30031-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SLC43A2 Polyclonal antibody (30031-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

  • WB analysis using 30031-1-AP

    WB analysis using 30031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of human lung cancer using 30031-1-AP

    IF Staining of human lung cancer using 30031-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using SLC43A2 antibody (30031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC43A2 Polyclonal antibody

SLC43A2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 30031-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), ELISA

别名

LAT4, L-type amino acid transporter 4, PP7664

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in37°C incubated mouse kidney tissue, mouse kidney tissue, mouse liver tissue, mouse liver tissue (37℃ incubated), rat liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy SLC43A2 Ready-To-Use IHC Kit. SLC43A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30031-1-AP targets SLC43A2 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31341

Product name: Recombinant human SLC43A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 516-569 aa of BC027923

Sequence: WVNVGLLLLSLLGFCLPLYLICYRRQLERQLQQRQEDDKLFLKINGSSNQEAFV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 43, member 2
别名 LAT4, L-type amino acid transporter 4, PP7664
计算分子量 63 kDa
观测分子量 48-53 kDa
GenBank蛋白编号BC027923
基因名称 SLC43A2
Gene ID (NCBI) 124935
RRIDAB_3086213
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N370
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC43A2 (solute carrier family 43 member 2, also known as LAT4) is a multi-pass transmembrane protein that mediates sodium- and chloride-independent uptake of large neutral amino acids, including methionine, leucine and phenylalanine. Highly expressed in kidney, placenta and liver, it supplies essential amino acids to support fetal growth, protein synthesis and one-carbon metabolism. Over-expressed in head-and-neck, liver and other cancers, SLC43A2-driven methionine influx maintains methylation reactions and YAP activity, promoting tumor proliferation and chemo-resistance . Pharmacological inhibition of SLC43A2 sensitizes tumors to therapy, highlighting its potential as a metabolic target. (PMID: 39747898; 15659399; 30379325)

实验方案

Product Specific Protocols
IHC protocol for SLC43A2 antibody 30031-1-APDownload protocol
WB protocol for SLC43A2 antibody 30031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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