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SLC26A8 Polyclonal antibody

SLC26A8 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 12776-1-AP

Print datasheet

Synonyms

Anion exchange transporter, SLC26A8, TAT1, Testis anion transporter 1

验证数据展示

  • WB analysis using 12776-1-AP

    WB analysis using 12776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12776-1-AP (SLC26A8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 12776-1-AP

    IF Staining of mouse testis using 12776-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12776-1-AP (SLC26A8 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IF detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12776-1-AP targets SLC26A8 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF-P, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SLC26A8 fusion protein Ag3472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 26, member 8
Synonyms Anion exchange transporter, SLC26A8, TAT1, Testis anion transporter 1
Calculated Molecular Weight 970 aa, 109 kDa
Observed Molecular Weight 62, 86 and 97 kDa
GenBank Accession NumberBC025408
Gene Symbol SLC26A8
Gene ID (NCBI) 116369
RRIDAB_2877880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC26A8, also named as TAT1, acts as a DIDS-sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been reported that SLC26A8 is a sulfate transporter specifically expressed in spermatocytes and spermatids, playing a key role in spermatogenesis. There are several isoforms of SLC26A8 and 12776-1-AP antibody detects 62, 86 and 97 kDa protein in SDS-PAGE. (PMID: 11278976; 11834742)

实验方案

Product Specific Protocols
WB protocol for SLC26A8 antibody 12776-1-APDownload protocol
IF protocol for SLC26A8 antibody 12776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease

Authors - Kun Mei
View Article