Recombinant Rat gp130/IL6ST protein (hFc Tag)
种属
Rat
纯度
>90 %, SDS-PAGE
标签
hFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Rat gp130 protein Gln23-Glu618 (Accession# NP_001008725.2) with a human IgG1 Fc tag at the C-terminus. |
| 基因ID | 25205 |
| 蛋白编号 | NP_001008725.2 |
| 预测分子量 | 93.7 kDa |
| SDS-PAGE | 100-130 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC). These cytokines signal through the gp130/Jak/STAT pathway. Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells.
参考文献:
1. T Taga, et al. (1997) Review Annu Rev Immunol. 15:797-819. 2. F A Montero-Julian, et al. (1997) Clin Cancer Res. 3(8):1443-51. 3. P C Heinrich, et al. (1998) Biochem J.334 ( Pt 2)(Pt 2):297-314. 4. J S Silver, et al. (2010) Review J Leukoc Biol. 88(6):1145-56.
