Recombinant Rat E-Cadherin protein (His Tag)

种属

Rat

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg1229

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Synonyms

Cadherin-1, CD324, Cdh1, E-Cad/CTF1, E-Cad/CTF2



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Rat E-Cadherin protein Gln24-Ala713 (Accession# Q9R0T4) with a His tag at the C-terminus.
基因ID 83502
蛋白编号 Q9R0T4
预测分子量 77.9 kDa
SDS-PAGE 70-85 kDa and 100 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.

参考文献:

1. D Riethmacher, et al. (1995) Proc Natl Acad Sci U S A. 92(3):855-9. 2. F van Roy, et al. (2008) Cell Mol Life Sci. 65(23):3756-88. 3. Weina Yu, et al. (2019) Front Oncol. 79:989.