Recombinant Rat Angiotensinogen/AGT protein (rFc Tag) (HPLC verified)
种属
Rat
纯度
>90 %, SDS-PAGE
>90 %, SEC-HPLC
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Rat Angiotensinogen protein Asp25-Val477 (Accession# P01015) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 24179 |
| 蛋白编号 | P01015 |
| 预测分子量 | 75.8 kDa |
| SDS-PAGE | 75-90 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Angiotensinogen (AGT) is a key component of the renin-angiotensin system (RAS), which plays a crucial role in blood pressure regulation and cardiovascular homeostasis Angiotensinogen is expressed in various tissues, including the liver, kidneys, and brain. In rats, the expression of angiotensinogen can be influenced by various factors such as stress, diet, and hormonal changes. For example, angiotensinogen expression in the brain can be affected by dehydration and salt loading, leading to changes in blood pressure and fluid balance. Transgenic rats with ubiquitous expression of angiotensinogen have been developed to study the role of the protective arm of the RAS in cardio-renal diseases.
参考文献:
1. Ranasinghe P, Addison ML, et al. (2022) J Am Heart Assoc. 11(20):e027694. 2. Uijl E, Mirabito Colafella KM, et al. (2019) Hypertension. 73(6):1249-1257. 3. Colόn NS, Pantho AF, et al. (2022) Pregnancy Hypertens. 227:1-5. 4. Ye D, Cruz-López EO, et al.(2024) Hypertension. 81(7):1491-1499.
