验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
67973-1-Ig targets RPA1 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, Rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag28239 Product name: Recombinant human RPA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-317 aa of BC018126 Sequence: MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKAAGPSLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKIANKQFTAVKNDYEMTFNNETSVMPCEDDHHLPTVQFDFTGIDDLENKSKDSLV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | replication protein A1, 70kDa |
| 别名 | HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70 |
| 计算分子量 | 616 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC018126 |
| 基因名称 | RPA1 |
| Gene ID (NCBI) | 6117 |
| RRID | AB_2918723 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P27694 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPA1 antibody 67973-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |