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RPA1 Monoclonal antibody

RPA1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G3F11

Cat No : 67973-1-Ig

Print datasheet

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

验证数据展示

  • WB analysis using 67973-1-Ig

    WB analysis using 67973-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67973-1-Ig (RPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

经过测试的应用

Positive WB detected inHCT 116 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67973-1-Ig targets RPA1 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, ELISA
Cited ApplicationsIF
Tested Reactivity Human, Rat
Cited Reactivityhuman
Immunogen RPA1 fusion protein Ag28239 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication protein A1, 70kDa
Synonyms HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70
Calculated Molecular Weight 616 aa, 68 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC018126
Gene Symbol RPA1
Gene ID (NCBI) 6117
RRIDAB_2918723
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPA1 antibody 67973-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.

Authors - Shaozu Fu
View Article