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验证数据展示

  • WB analysis using 12473-1-AP

    WB analysis using 12473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12473-1-AP

    IP experiment of mouse brain using 12473-1-AP

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug.

  • IP experiment of rat brain using 12473-1-AP

    IP experiment of rat brain using 12473-1-AP

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug.

  • IHC staining of mouse brain using 12473-1-AP

    IHC staining of mouse brain using 12473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 12473-1-AP

    IHC staining of mouse pancreas using 12473-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RIT2 Polyclonal antibody

RIT2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12473-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

GTP binding protein Rit2, Ras like without CAAX 2, RIBA, RIN, RIT2, ROC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12473-1-AP targets RIT2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3160

Product name: Recombinant human RIT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC018060

Sequence: MEVENEASCSPGSASGGSREYKVVMLGAGGVGKSAMTMQFISHQFPDYHDPTIEDAYKTQVRIDNEPAYLDILDTAGQAEFTAMREQYMRGGEGFIICYSVTDRQSFQEAAKFKELIFQVRHTYEIPLVLVGNKIDLEQFRQVSTEEGLSLAQEYNCGFFETSAALRFCIDDAFHGLVREIRKKESMPSLMEKKLKRKDCLWKKLKGSLKKKRENMT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-like without CAAX 2
别名 GTP binding protein Rit2, Ras like without CAAX 2, RIBA, RIN, RIT2, ROC2
计算分子量 217 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018060
基因名称 RIT2
Gene ID (NCBI) 6014
RRIDAB_3669140
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99578
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).

实验方案

Product Specific Protocols
WB protocol for RIT2 antibody 12473-1-APDownload protocol
IHC protocol for RIT2 antibody 12473-1-APDownload protocol
IP protocol for RIT2 antibody 12473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols