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Conjugate
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验证数据展示

  • WB analysis using 85347-1-RR (same clone as 85347-1-PBS)

    WB analysis using 85347-1-RR (same clone as 85347-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse kidney using 85347-1-RR (same clone as 85347-1-PBS)

    WB analysis of mouse kidney using 85347-1-RR (same clone as 85347-1-PBS)

    Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85347-1-RR

    Affinity and Kinetic Characterization of 85347-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85347-1-RR against Human RIT1 were performed. The affinity constant is 0.505 nM.

RIT1 Recombinant monoclonal antibody, PBS Only

RIT1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85347-1-PBS

产品说明书

CloneNo. 242593E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

RIBB, Ras like without CAAX protein 1, Ras like without CAAX 1, Ras like protein expressed in many tissues, GTP binding protein Rit1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85347-1-PBS targets RIT1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25636

Product name: Recombinant human RIT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-219 aa of BC104186

Sequence: QLRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSPFRKKKDSVT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Ras-like without CAAX 1
别名 RIBB, Ras like without CAAX protein 1, Ras like without CAAX 1, Ras like protein expressed in many tissues, GTP binding protein Rit1
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC104186
基因名称 RIT1
Gene ID (NCBI) 6016
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92963
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).

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