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验证数据展示

  • WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    WB result of RASEF antibody (67159-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RASEF transfected LNCaP cells. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RASEF antibody included
  • IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RASEF antibody included
  • Featured Product
  • KD/KO验证

RASEF Monoclonal antibody, PBS Only

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67159-1-PBS

产品说明书

CloneNo. 1C2B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

产品信息

67159-1-PBS targets RASEF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2149

Product name: Recombinant human RASEF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 354-740 aa of BC023566

Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAS and EF-hand domain containing
别名 RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45
计算分子量 740 aa, 83 kDa
观测分子量 90 kDa
GenBank蛋白编号BC023566
基因名称 RASEF
Gene ID (NCBI) 158158
RRIDAB_2882456
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZ41
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

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