验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, human placenta tissue, MCF-7 cells, RT-4 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15926-1-AP targets QRSL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8756 Product name: Recombinant human QRSL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-528 aa of BC014389 Sequence: GIPKEYLVPELSSEVQSLWSKAADLFESEGAKVIEVSLPHTSYSIVCYHVLCTSEVASNMARFDGLQYGHRCDIDVSTEAMYAATRREGFNDVVRGRILSGNFFLLKENYENYFVKAQKVRRLIANDFVNAFNSGVDVLLTPTTLSEAVPYLEFIKEDNRTRSAQDDIFTQAVNMAGLPAVSIPVALSNQGLPIGLQFIGRAFCDQQLLTVAKWFEKQVQFPVIQLQELMDDCSAVLENEKLASVSLKQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 |
| 别名 | EC:6.3.5.7, GatA, Glu-AdT subunit A, Glutaminyl-tRNA synthase-like protein 1, Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial |
| 计算分子量 | 528 aa, 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC014389 |
| 基因名称 | QRSL1 |
| Gene ID (NCBI) | 55278 |
| RRID | AB_10638908 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0R6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
QRSL1 is a mitochondrial glutamyl‑tRNA(Gln) amidotransferase subunit A in humans, encoded by nuclear‑located QRSL1 gene. It mediates Gln‑tRNA(Gln) biosynthesis via transamidation, supporting mitochondrial translation and oxidative phosphorylation function. Biallelic pathogenic QRSL1 variants trigger combined oxidative phosphorylation deficiency 40 (COXPD40), a severe autosomal‑recessive mitochondrial disorder with cardiomyopathy, adrenal insufficiency and multi‑organ injury in infants. (PMID: 35894854)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for QRSL1 antibody 15926-1-AP | Download protocol |
| WB protocol for QRSL1 antibody 15926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
