Recombinant Pig IL-10 protein (rFc Tag)
种属
Pig
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Pig IL-10 protein Ser19-Asn175 (Accession# P05109) with a rabbit IgG Fc tag at the N-terminus. |
| 基因ID | 397106 |
| 蛋白编号 | Q29055 |
| 预测分子量 | 45.3 kDa |
| SDS-PAGE | 44-52 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
参考文献:
1. Dinarello CA. et al. (1996). Blood. 87: 2095-147. 2. Bird S. et al. (2002). Cytokine Growth Factor Rev. 13: 483-502. 3. Xu J. et al. (2013). PLoS One. 21;8(5):e63654. 4. McCarty S. (2014). Cardiol Rev. 22: 176-81.
