验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
22799-1-AP targets PYGO1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | PYGO1 fusion protein Ag18814 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pygopus homolog 1 (Drosophila) |
| 别名 | PYGO1, Pygopus homolog 1, pygopus homolog 1 (Drosophila) |
| 计算分子量 | 419 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC146572 |
| 基因名称 | PYGO1 |
| Gene ID (NCBI) | 26108 |
| RRID | AB_2879171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3Y4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PYGO1 antibody 22799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Heart Circ Physiol Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism. | ||
Hum Cell Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway |