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验证数据展示

  • WB analysis of SKOV-3 using 66885-1-Ig (same clone as 66885-1-PBS)

    WB analysis of SKOV-3 using 66885-1-Ig (same clone as 66885-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 66885-1-Ig (PRMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

    IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT2 antibody included
  • IF Staining of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

    IF Staining of HeLa using 66885-1-Ig (same clone as 66885-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRMT2 antibody (66885-1-Ig, Clone: 1F1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

    IHC staining of human thyroid cancer using 66885-1-Ig (same clone as 66885-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66885-1-Ig (PRMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66885-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT2 antibody included

PRMT2 Monoclonal antibody, PBS Only

PRMT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66885-1-PBS

产品说明书

CloneNo. 1F1B9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HMT1, HRMT1L1, PRMT2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRMT2 Ready-To-Use IHC Kit. PRMT2 primary antibody included.

产品信息

66885-1-PBS targets PRMT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag1806

Product name: Recombinant human PRMT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 134-433 aa of BC000727

Sequence: ESLTDKVILDVGCGTGIISLFCAHYARPRAVYAVEASEMAQHTGQLVLQNGFADIITVYQQKVEDVVLPEKVDVLVSEWMGTCLLFEFMIESILYARDAWLKEDGVIWPTMAALHLVPCSADKDYRSKVLFWDNAYEFNLSALKSLAVKEFFSKPKYNHILKPEDCLSEPCTILQLDMRTVQISDLETLRGELRFDIRKAGTLHGFTAWFSVHFQSLQEGQPPQVLSTGPFHPTTHWKQTLFMMDDPVPVHTGDVVTGSVVLQRNPVWIRHMSVALSWAVTSRQDPTSQKVGEKVFPIWR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein arginine methyltransferase 2
别名 HMT1, HRMT1L1, PRMT2
计算分子量 49 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC000727
基因名称 PRMT2
Gene ID (NCBI) 3275
RRIDAB_2882217
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55345
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT2 (also known as HRMT1L1) is a member of the arginine methyltransferase family which catalyzes arginine methylation and regulates diverse cellular processes, including transcription, translation, DNA repair, and RNA processing. Recently PRMT2 has been identified as a pro-tumorigenic factor in malignant gliomas.

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