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PIR Monoclonal antibody

PIR Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2A10E4

Cat No : 67516-1-Ig

Print datasheet

Synonyms

PIR, Pirin

验证数据展示

  • WB analysis of HSC-T6 using 67516-1-Ig

    WB analysis of HSC-T6 using 67516-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67516-1-Ig (PIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHSC-T6 cells, HeLa cells, K-562 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67516-1-Ig targets PIR in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,ELISA
Tested Reactivity Human, mouse, rat
Immunogen PIR fusion protein Ag29969 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pirin (iron-binding nuclear protein)
Synonyms PIR, Pirin
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002517
Gene Symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2882737
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

实验方案

Product Specific Protocols
WB protocol for PIR antibody 67516-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols