验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, HeLa cells, K-562 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67516-1-Ig targets PIR in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag29969 Product name: Recombinant human PIR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 46-230 aa of BC002517 Sequence: KGGRPGGFPDHPHRGFETVSYLLEGGSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIPKPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQKIEPHHTAVLGEGDSVQVEN 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pirin (iron-binding nuclear protein) |
| 别名 | PIR, Pirin |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC002517 |
| 基因名称 | PIR |
| Gene ID (NCBI) | 8544 |
| RRID | AB_2882737 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00625 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIR antibody 67516-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
