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验证数据展示

  • WB analysis using 85738-1-RR (same clone as 85738-1-PBS)

    WB analysis using 85738-1-RR (same clone as 85738-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85738-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

  • WB analysis of human peripheral blood platelets using 85738-1-RR (same clone as 85738-1-PBS)

    WB analysis of human peripheral blood platelets using 85738-1-RR (same clone as 85738-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85738-1-RR (PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    IF Staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CXCL4/PF4 antibody (85738-1-RR, Clone: 242741H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    IHC staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85738-1-RR (CXCL4/PF4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    IHC staining of mouse spleen using 85738-1-RR (same clone as 85738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85738-1-RR (CXCL4/PF4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85738-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02069-1

    Cytometric bead array standard curve of MP02069-1

    Cytometric bead array standard curve of MP02069-1, MOUSE CXCL4/PF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85738-1-PBS. Detection antibody: 85738-2-PBS. Standard: Eg3345. Range: 0.781-100 ng/mL.

Mouse CXCL4/PF4 Recombinant monoclonal antibody, PBS Only (Capture)

CXCL4/PF4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 85738-1-PBS

产品说明书

CloneNo. 242741H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

C-X-C motif chemokine 4, CXCL4, Pf4, PF-4, Platelet factor 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85738-1-PBS targets CXCL4/PF4 as part of a matched antibody pair:

MP02069-1: 85738-1-PBS capture and 85738-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg3345

Product name: Recombinant Mouse CXCL4/PF4 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 30-105 aa of NM_019932

Sequence: VTSAGPEESDGDLSCVCVKTISSGIHLKHITSLEVIKAGRHCAVPQLIATLKNGRKICLDRQAPLYKKVIKKILES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 platelet factor 4
别名 C-X-C motif chemokine 4, CXCL4, Pf4, PF-4, Platelet factor 4
计算分子量11kd
观测分子量 10-14 kDa
GenBank蛋白编号NM_019932
基因名称 Pf4
Gene ID (NCBI) 56744
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Z126
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.

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