验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27598-1-AP targets ORMDL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26253 Product name: Recombinant human ORMDL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of BC071833 Sequence: MNVGTAHSEVNPNTRVMNSRGIWLSYVLAIGLLHIVLLSIPFVSVPVVWTLTNLIHNMGMYIFLHTVKGTPFETPDQGKARLLTHWEQMDYGVQFTASRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ORM1-like 3 (S. cerevisiae) |
| 别名 | ORM1 like protein 3 |
| 计算分子量 | 153 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC071833 |
| 基因名称 | ORMDL3 |
| Gene ID (NCBI) | 94103 |
| RRID | AB_3742242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N138 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ORMDL3 (ORM1-like 3) is an endoplasmic-reticulum (ER)-resident transmembrane protein that negatively regulates serine palmitoyl-transferase (SPT), the first and rate-limiting enzyme of de-novo sphingolipid biosynthesis, thereby controlling cellular ceramide levels. (PMID: 31376405)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ORMDL3 antibody 27598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
