验证数据展示
经过测试的应用
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
28432-1-AP targets OLFM4 in IHC, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28914 Product name: Recombinant human OLFM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 60-233 aa of NM_006418 Sequence: LGSGGSVSQLFSNFTGSVDDRGTCQCSVSLPDTTFPVDRVERLEFTAHVLSQKFEKELSKVREYVQLISVYEKKLLNLTVRIDIMEKDTISYTELDFELIKVEVKEMEKLVIQLKESFGGSSEIVDQLEVEIRNMTLLVEKLETLDKNNVLAIRREIVALKTKLKECEASKDQN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | olfactomedin 4 |
| 别名 | Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362 |
| 计算分子量 | 57 kDa |
| GenBank蛋白编号 | NM_006418 |
| 基因名称 | OLFM4 |
| Gene ID (NCBI) | 10562 |
| RRID | AB_2918163 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UX06 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Olfactomedin-4 (OLFM4), also named as hGC-1 (human G-CSF-stimulated clone-1) or antiapoptotic protein GW112, is a secreted glycoprotein normally expressed in bone marrow, prostate, small intestine, stomach, colon and pancreas (PMID: 11867215; PMID: 22471589). OLFM4 has been found to be up-regulated in gastrointestinal cancer and inflammatory disease including inflammatory bowel disease and H. pylori infection (PMID: 20534456). OLFM4 is involved in cellular process such as apoptosis, cell adhesion and tumor growth (PMID: 22471589; 16566923; 15059901).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for OLFM4 antibody 28432-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Deciphering the Metabolic Impact and Clinical Relevance of N-Glycosylation in Colorectal Cancer through Comprehensive Glycoproteomic Profiling | ||