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验证数据展示

  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with OLFM4 antibody included
  • IHC staining of human small intestine using 28432-1-AP

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with OLFM4 antibody included

OLFM4 Polyclonal antibody

OLFM4 Polyclonal Antibody for IHC, ELISA
Cat No. 28432-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (2)

应用

IHC, ELISA and More (1)

别名

Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OLFM4 Ready-To-Use IHC Kit. OLFM4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28432-1-AP targets OLFM4 in IHC, IF, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 Human
文献引用反应性human, mouse
免疫原 OLFM4 fusion protein Ag28914 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 olfactomedin 4
别名 Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362
计算分子量 57 kDa
GenBank蛋白编号NM_006418
基因名称 OLFM4
Gene ID (NCBI) 10562
RRIDAB_2918163
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UX06
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Olfactomedin-4 (OLFM4), also named as hGC-1 (human G-CSF-stimulated clone-1) or antiapoptotic protein GW112, is a secreted glycoprotein normally expressed in bone marrow, prostate, small intestine, stomach, colon and pancreas (PMID: 11867215; PMID: 22471589). OLFM4 has been found to be up-regulated in gastrointestinal cancer and inflammatory disease including inflammatory bowel disease and H. pylori infection (PMID: 20534456). OLFM4 is involved in cellular process such as apoptosis, cell adhesion and tumor growth (PMID: 22471589; 16566923; 15059901).

实验方案

Product Specific Protocols
IHC protocol for OLFM4 antibody 28432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Adv Sci (Weinh)

Deciphering the Metabolic Impact and Clinical Relevance of N-Glycosylation in Colorectal Cancer through Comprehensive Glycoproteomic Profiling

Authors - Guobin Liu
View Article
mouseIF

Cell Rep

An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation

Authors - Ketty Sinigaglia
View Article