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验证数据展示

  • WB analysis using 85998-1-RR

    WB analysis using 85998-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85998-1-RR (OGFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 85998-1-RR

    IP experiment of COLO 320 using 85998-1-RR

    IP result of anti-OGFR (IP:85998-1-RR, 4ug; Detection:85998-1-RR 1:3000) with COLO 320 cells lysate 1920 ug.

  • IHC staining of human colon cancer using 85998-1-RR

    IHC staining of human colon cancer using 85998-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85998-1-RR (OGFR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of L02 using 85998-1-RR

    IF Staining of L02 using 85998-1-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using OGFR antibody (85998-1-RR, Clone: 250452E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85998-1-RR

    Affinity and Kinetic Characterization of 85998-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85998-1-RR against Human OGFR were performed. The affinity constant is 0.211 nM.

OGFR Recombinant monoclonal antibody

OGFR Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 85998-1-RR

产品说明书

CloneNo. 250452E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, A549 cells, L02 cells, HEK-293 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85998-1-RR targets OGFR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1671

Product name: Recombinant human OGFR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 358-658 aa of BC014137

Sequence: LERSQGDEAGGHGEDRPEPLSPKESKKRKLELSRREQPPTEPGPQSASEVEKIALNLEGCALSQGSLRTGTQEVGGQDPGEAVQPCRQPLGARVADKVRKRRKVDEGAGDSAAVASGGAQTLALAGSPAPSGHPKAGHSENGVEEDTEGRTGPKEGTPGSPSETPGPSPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAESPSETPGPRPAGPAGDEPAESPSETPGPRPAGPAGDEPAESPSETPGPSPAGPTRDEPAKAGEAAELQDAEVESSAKSGKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 opioid growth factor receptor
别名 Opioid growth factor receptor, Protein 7 60, Protein 7-60, Zeta type opioid receptor, Zeta-type opioid receptor
计算分子量 73 kDa
观测分子量110 kDa
GenBank蛋白编号BC014137
基因名称 OGFR
Gene ID (NCBI) 11054
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for OGFR antibody 85998-1-RRDownload protocol
IHC protocol for OGFR antibody 85998-1-RRDownload protocol
IP protocol for OGFR antibody 85998-1-RRDownload protocol
WB protocol for OGFR antibody 85998-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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