ChromoTek Nano-CaptureLigand™ mouse IgG2b, Fc-specific VHH, biotinylated

The Nano-CaptureLigand mouse IgG2b captures and immobilizes mouse IgG2b antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to mouse IgG2b antibodies with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Mouse IgG2b

Cat No : smsG2bB-1

Synonyms

Nano-CaptureLigand™ mouse IgG2b, Fc domain, mouse IgG2b, mouse immunoglobulin Gamma 2b



Product Information

The Nano-CaptureLigand mouse IgG2b captures and immobilizes mouse IgG2b antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to mouse IgG2b antibodies with high affinity and in a site-directed manner.

DescriptionThe next level of antibody immobilization to BLI and SPR biosensors:

• Gentle, site-directed and Fc-specific immobilization of mouse IgG2b antibodies and Fc-fragments to streptavidin coated biosensors without biotinylation of the antibody

• Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

• High affinity for stable baseline without significant dissociation

• >10x regeneration for multiple reuse

• High specificity enables mouse IgG2b antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

ApplicationsBLI, SPR, ELISA
HostAlpaca
ReactivityFc-fragment of mouse IgG2b
No cross-reactivity to mouse IgG1, Ig2a, Ig2c, IgG3; human IgG1, IgG2, IgG3, IgG4, IgA; rat IgG1, IgG2a, IgG2b; sera from goat, human, macaque (cynomolgus monkey), sheep
ConjugateBiotin
Physical StateLiquid
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD) KD= 0.2 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
RRIDAB_2848187
NoteThis product is for research use only, not for diagnostic or therapeutic use