ChromoTek Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Human, Rabbit

Cat No : shurbGB-1

Synonyms

immunoglobulin, human IgG, Fc domain, Rabbit IgG



Product Information

The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.

DescriptionThe next level of antibody immobilization to BLI and SPR biosensors

• Site-directed and specific immobilization of the Fc-fragment of human IgG and rabbit IgG to streptavidin coated biosensors without biotinylation of the antibody

• Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

• High affinity for stable baseline without significant dissociation

• >10x regeneration for multiple reuse

• High specificity enables Fc-fragment of human IgG and rabbit IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

ApplicationsBLI, SPR, ELISA
HostAlpaca
ReactivityHuman IgG1, IgG2, IgG3, and IgG4 Fc-fragments
Rabbit IgG Fc-fragment
ConjugateBiotin
Physical StateLiquid
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD) KD:Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM
Rabbit IgG: 0.2 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
RRIDAB_2848185
NoteThis product is for research use only, not for diagnostic or therapeutic use