验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
20209-1-AP targets NARFL-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear prelamin A recognition factor-like |
| 别名 | CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf |
| 计算分子量 | 53 kDa |
| 观测分子量 | 26-28 kDa |
| GenBank蛋白编号 | NM_022493 |
| 基因名称 | NARFL |
| Gene ID (NCBI) | 64428 |
| RRID | AB_10694435 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H6Q4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NARFL-Specific antibody 20209-1-AP | Download protocol |
| WB protocol for NARFL-Specific antibody 20209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
