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验证数据展示

  • Single Cell Sequencing experiment G66095-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66095-1-5C on Resting PBMC

    G66095-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66095-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66095-1-5C on PBMC treated with 4hr LPS + TI

    G66095-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human Lamin B1 (3C10G12)

Lamin B1 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66095-1-5C

产品说明书

CloneNo. 3C10G12

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

Single Cell (Intra)

别名

ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66095-1-5C targets Lamin B1 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 Lamin B1 fusion protein Ag20522 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human Lamin B1 (3C10G12)
别名 ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1
计算分子量 66 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_3673943
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTCGTACTATACTAACCCCATATAAGAAA
条形码序列TCGTACTATACTAAC
形式 Liquid
UNIPROT IDP20700
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol