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Recombinant Mouse tPA protein (rFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg3025

Print datasheet

Synonyms

EC:3.4.21.68, Plat, Tissue-type plasminogen activator, Tissue-type plasminogen activator chain A, Tissue-type plasminogen activator chain B

验证数据展示

  • Purity of Recombinant Mouse tPA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse tPA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse tPA protein Leu18-Gln559 (Accession# P11214) with a rabbit IgG Fc tag at the C-terminus.
基因ID 18791
蛋白编号 P11214
预测分子量 87.2 kDa
SDS-PAGE 27-30 kDa, 35-37 kDa and 58-68 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

tPA (Tissue-type plasminogen activator), also known as Plat, is a serine proteinase found in the intravascular space and the CNS. In the brain tPA is found in the endothelial cell–basement membrane–astrocyte interface, where it modulates cerebrovascular tone and the permeability of the blood-brain barrier. tPA is a key component in the dissolution of blood clots, primarily by catalyzing the conversion of plasminogen to plasmin, the primary enzyme involved in dissolving blood clots.

参考文献:

1. Echeverry R, et al. (2010). J Clin Invest. Jun;120(6):2194-205. 
2. Bugge, T. H., et al. (1996). Proc Natl Acad Sci U S A. Jun 11;93(12):5899-904.
3. Sashindranath M, et al. (2012). Brain. Nov;135(Pt 11):3251-64. 
4. Collen D, et al. (2004). J Thromb Haemost. Apr;2(4):541-6.