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Recombinant Mouse C5 protein (rFc Tag) (HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg3332

Print datasheet

Synonyms

C5a anaphylatoxin, Complement C5, Complement C5 alpha chain, Complement C5 alpha' chain, Complement C5 beta chain

验证数据展示

  • Purity of Recombinant Mouse C5 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse C5 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse C5 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse C5 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse C5 protein Asn679-Arg755 (Accession# P06684) with a rabbit IgG Fc tag at the C-terminus.
基因ID 15139
蛋白编号 P06684
预测分子量 35.2 kDa
SDS-PAGE 36-45 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections.

参考文献:

1.Ajona, D. et al. (2024). Semin immunol. 77:101921.
2.Azoulay, E. et al. (2024). Intens care med. 50(11):1791-1803.
3.Merle, NS. et al. (2024). Eur j immunol. 54(10):e2350820.
4.Dunkelberger, JR. et al. (2010). Cell res. 20(1):34-50.
5.DiScipio, RG. et al. (1983). J biol chem. 258(17):10629-36.