Recombinant Mouse CD200 protein (hFc Tag)(HPLC verified)
种属
Mouse
纯度
>95 %, SDS-PAGE
>90 %, SEC-HPLC
标签
hFc Tag
生物活性
EC50: 8-32 ng/mL
验证数据展示
产品信息
| 纯度 | >95 %, SDS-PAGE >90 %, SEC-HPLC |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Immobilized Mouse CD200R1 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD200 (hFc tag) with a linear range of 8-32 ng/mL. |
| 来源 | HEK293-derived Mouse CD200 protein Gln31-Gly232 (Accession# O54901) with a human IgG1 Fc tag at the C-terminus. |
| 基因ID | 17470 |
| 蛋白编号 | O54901 |
| 预测分子量 | 48.5 kDa |
| SDS-PAGE | 60-68 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells. CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage. The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses.
参考文献:
1.G W McCaughan, et al. (1987) Immunogenetics. 25(5):329-35. 2.G J Wright, et al. (2000) Immunity. 13(2):233-42. 3.Reginald Gorczynski, et al. (2004) J Immunol. 172(12):7744-9. 4.T P Rygiel, et al. (2012) Oncogene. 31(24):2979-88.
