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Recombinant Mouse Angiotensinogen protein (rFc Tag) (HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg2763

Print datasheet

Synonyms

Agt, Serpina8

验证数据展示

  • Purity of Recombinant Mouse Angiotensinogen was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Angiotensinogen was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Angiotensinogen was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Angiotensinogen was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse Angiotensinogen protein Asp25-Val477 (Accession# P11859) with a rabbit IgG Fc tag at the C-terminus.
基因ID 11606
蛋白编号 P11859
预测分子量 75.6 kDa
SDS-PAGE 70-85 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. AGT is an essential component of the RAS. AGT is cleaved by renin enzyme to produce angiotensin 1 (Ang I) when blood pressure is reduced, and AGT-converting enzyme (ACE) splits Ang I to generate angiotensin 2 (Ang II). RAS plays an important role in maintaining fluid and electrolyte homeostasis, and RAS irregulated expression leads to kidney disease, heart failure, hypertension, and diabetes.

参考文献:

1. Matsusaka T, et al. (2012). J Am Soc Nephrol. 23(7):1181-9. 
2. Lu H, et al. (2016). Hypertens Res.39(7):492-500. 
3. Li S, et al. (2021). Mol Med Rep. 23(3):193.
4. Wegman-Ostrosky T, et al. (2015). J Renin Angiotensin Aldosterone Syst.16(2):227-33.
5. Ames MK, et al. (2019). J Vet Intern Med. 33(2):363-382. 
6. Li XC, et al. (2017). 125(Pt A):21-38.