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验证数据展示

  • WB analysis using 67820-1-Ig

    WB analysis using 67820-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67820-1-Ig (MVK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MVK Monoclonal antibody

MVK Monoclonal Antibody for WB, ELISA
Cat No. 67820-1-Ig

产品说明书

CloneNo. 2F1D3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, ELISA

别名

FLJ96772, LRBP, mevalonate kinase, MK, MVK, MVLK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, U2OS cells, HepG2 cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67820-1-Ig targets MVK in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag28887

Product name: Recombinant human MVK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-396 aa of BC016140

Sequence: MLSEVLLVSAPGKVILHGEHAVVHGKVALAVSLNLRTFLRLQPHSNGKVDLSLPNIGIKRAWDVARLQSLDTSFLEQGDVTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQRALPSLDIVVWSELPPGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLELINKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQGKISSLKRSPALQILLTNTKVPRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELIDMNQHHLNALGVGHASLDQLCQVTRARGLHSKLTGAGGGGCGITLLKPGLEQPEVEATKQALTSCGFDCLETSIGAPGVSIHSATSLDSRVQQALDGL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mevalonate kinase
别名 FLJ96772, LRBP, mevalonate kinase, MK, MVK, MVLK
计算分子量 396 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC016140
基因名称 MVK
Gene ID (NCBI) 4598
RRIDAB_2918583
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03426
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

实验方案

Product Specific Protocols
WB protocol for MVK antibody 67820-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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