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MUC16/CA125 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 97.7-6250 pg/mL

Cat no : MP03433-1

Synonyms

MUC16, MUC16,CA125, CA 125, CA125, MUC 16

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03433-1, Human MUC16/CA125 Recombinant Matched Antibody Pair - PBS only. 86537-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6451. 86537-4-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

    Sandwich ELISA standard curve of MP03433-1

    Sandwich ELISA standard curve of MP03433-1, Human MUC16/CA125 Recombinant Matched Antibody Pair - PBS only. 86537-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6451. 86537-4-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

  • Plasma of six individual healthy human donors was measured. The MUC16/CA125 concentration of detected samples was determined to be 203.7 pg/mL with a range of 113.5-313.1 pg/mL.

    Sandwich ELISA sample test of MP03433-1

    Plasma of six individual healthy human donors was measured. The MUC16/CA125 concentration of detected samples was determined to be 203.7 pg/mL with a range of 113.5-313.1 pg/mL.

  • Human milk of six individual healthy human donors was measured. The MUC16/CA125 concentration of detected samples was determined to be 3,418.5 pg/mL with a range of 2,085.2-6,830.3 pg/mL.

    Sandwich ELISA sample test of MP03433-1

    Human milk of six individual healthy human donors was measured. The MUC16/CA125 concentration of detected samples was determined to be 3,418.5 pg/mL with a range of 2,085.2-6,830.3 pg/mL.

  • The mean MUC16/CA125 concentration was determined to be 522.1 pg/mL in HeLa cell culture supernatant.

    Sandwich ELISA sample test of MP03433-1

    The mean MUC16/CA125 concentration was determined to be 522.1 pg/mL in HeLa cell culture supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP03433-1 targets MUC16/CA125 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 97.7-6250 pg/mL (Sandwich ELISA);

Capture antibody: MUC16 Recombinant monoclonal antibody, PBS Only (Capture) 86537-3-PBS (260551E1). 100 μg. Concentration 1 mg/ml.

Detection antibody: MUC16 Recombinant monoclonal antibody, PBS Only (Detector) 86537-4-PBS (260551C10). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg6451

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86537-3-PBS
Tested Reactivity human
Immunogen MUC16 fusion protein Eg6451
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mucin 16, cell surface associated
Synonyms
GenBank accession numberNM_024690.2
Gene symbol MUC16
UniprotQ8WXI7
Gene ID (NCBI) 94025
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86537-4-PBS
Tested Reactivity human
Immunogen MUC16 fusion protein Eg6451
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mucin 16, cell surface associated
Synonyms
GenBank accession numberNM_024690.2
Gene symbol MUC16
UniprotQ8WXI7
Gene ID (NCBI) 94025
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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