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MUC13 Polyclonal antibody

MUC13 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29832-1-AP

Print datasheet

Synonyms

DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC

验证数据展示

  • WB analysis using 29832-1-AP

    WB analysis using 29832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29832-1-AP (MUC13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HT-29 cells, SW480 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29832-1-AP targets MUC13 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen MUC13 fusion protein Ag31090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mucin 13, cell surface associated
Synonyms DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC
Calculated Molecular Weight 55 kDa
Observed Molecular Weight70-120 kDa
GenBank Accession NumberNM_033049
Gene Symbol MUC13
Gene ID (NCBI) 56667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3R2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

实验方案

Product Specific Protocols
WB protocol for MUC13 antibody 29832-1-APDownload protocol
IHC protocol for MUC13 antibody 29832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols