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验证数据展示

  • WB analysis using 29832-1-AP

    WB analysis using 29832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29832-1-AP (MUC13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC13 antibody included
  • IHC staining of human colon cancer using 29832-1-AP

    IHC staining of human colon cancer using 29832-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29832-1-AP (MUC13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MUC13 antibody included

MUC13 Polyclonal antibody

MUC13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29832-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HT-29 cells, SW480 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MUC13 Ready-To-Use IHC Kit. MUC13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29832-1-AP targets MUC13 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag31090

Product name: Recombinant human MUC13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 208-421 aa of NM_033049

Sequence: STCKKGKVFPGKISVTVSETFDPEEKHSMAYQDLHSEITSLFKDVFGTSVYGQTVILTVSTSLSPRSEMRADDKFVNVTIVTILAETTSDNEKTVTEKINKAIRSSSSNFLNYDLTLRCDYYGCNQTADDCLNGLACDCKSDLQRPNPQSPFCVASSLKCPDACNAQHKQCLIKKSGGAPECACVPGYQEDANGNCQKCAFGYSGLDCKDKFQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mucin 13, cell surface associated
别名 DRCC1, FLJ20063, MUC 13, MUC13, Mucin 13, RECC
计算分子量 55 kDa
观测分子量70-120 kDa
GenBank蛋白编号NM_033049
基因名称 MUC13
Gene ID (NCBI) 56667
RRIDAB_2923611
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3R2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mucins (MUC) are highly glycosylated proteins that are abundantly expressed in mammalian epithelial cells. Mucin 13 (MUC13) is a member of the transmembrane glycoprotein mucins which is normally expressed in the large intestine, trachea, kidney, small intestine, and gastric epithelium (PMID: 22027689). MUC13 is involved in the tumorigenesis of multiple malignancies, including pancreatic cancer, colorectal cancer, renal cancer and lung cancer (PMID: 22027689; PMID: 31427737; PMID: 28205224; PMID: 34966453).

实验方案

Product Specific Protocols
IHC protocol for MUC13 antibody 29832-1-APDownload protocol
WB protocol for MUC13 antibody 29832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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