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验证数据展示

  • IHC staining of mouse brain using 30142-1-AP

    IHC staining of mouse brain using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30142-1-AP

    IHC staining of mouse brain using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 30142-1-AP

    IHC staining of rat kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 30142-1-AP

    IHC staining of rat kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30142-1-AP

    IHC staining of mouse kidney using 30142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 30142-1-AP

    IF Staining of mouse kidney using 30142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 30142-1-AP

    IF Staining of mouse kidney using 30142-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LPHN1 Polyclonal antibody

LPHN1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30142-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

CIRL-1, CIRL1, CIRL 1, Calcium-independent alpha-latrotoxin receptor 1, Adhesion G protein-coupled receptor L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, rat kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30142-1-AP targets LPHN1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LPHN1 fusion protein Ag31093 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 latrophilin 1
别名 CIRL-1, CIRL1, CIRL 1, Calcium-independent alpha-latrotoxin receptor 1, Adhesion G protein-coupled receptor L1
计算分子量 163 kDa
GenBank蛋白编号NM_001008701
基因名称 LPHN1
Gene ID (NCBI) 22859
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Latrophilin-1 (LPHN1) was isolated as the main high-affinity receptor for α-latrotoxin from black widow spider venom, a powerful presynaptic secretagogue (PMID: 30967757). aGPCR latrophilin 1 (ADGRL1/LPHN1) is involved in controlling energy balance and food intake. Although this receptor is present in adipocytes and many neurons, where it is essential for axon growth cone formation and neuronal. It is present in Adipocytes and many neurons, where it is essential for Axon growth cone formation and neuronal function (PMID: 38664368).

实验方案

Product Specific Protocols
IHC protocol for LPHN1 antibody 30142-1-APDownload protocol
IF protocol for LPHN1 antibody 30142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols