验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83415-3-RR targets Cytokeratin 77 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21942 Product name: Recombinant human KRT77 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 490-578 aa of BC122558 Sequence: QNSQVSVNGGAGGGGSYGSGGYGGGSGGGYGGGRSYRGGGARGGSGGGYGSGCGGGGGSYGGSGRSGRGSSRVQIIQTSTNTSHRRILE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | keratin 77 |
| 别名 | KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B |
| 计算分子量 | 578 aa, 62 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC122558 |
| 基因名称 | KRT77 |
| Gene ID (NCBI) | 374454 |
| RRID | AB_3671064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q7Z794 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 77 antibody 83415-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
