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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82972-1-RR (same clone as 82972-1-PBS)

    WB analysis using 82972-1-RR (same clone as 82972-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82972-1-RR (KRT1-specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82972-1-RR (same clone as 82972-1-PBS)

    FC experiment of A431 using 82972-1-RR (same clone as 82972-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human KRT1-specific (82972-1-RR, Clone:230261F9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP)(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82972-1-PBS in a different storage buffer formulation.

Cytokeratin 1-specific Recombinant antibody, PBS Only

Cytokeratin 1-specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82972-1-PBS

产品说明书

CloneNo. 230261F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

KRT1-specific, Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82972-1-PBS targets Cytokeratin 1-specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 1
别名 KRT1-specific, Cytokeratin 1, Cytokeratin-1, Cytokeratin1, Cytokeratin,Keratin
计算分子量 66 kDa
观测分子量 67 kDa
GenBank蛋白编号NM_006121
基因名称 KRT1
Gene ID (NCBI) 3848
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04264
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.