×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87495-1-RR (same clone as 87495-1-PBS)

    WB analysis using 87495-1-RR (same clone as 87495-1-PBS)

    RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 87495-1-RR (ITB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87495-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 87495-1-RR (same clone as 87495-1-PBS)

    IF Staining of RAW 264.7 using 87495-1-RR (same clone as 87495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Integrin Beta 1/CD29 antibody (87495-1-RR, Clone: 252764F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87495-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87495-1-RR (same clone as 87495-1-PBS)

    IHC staining of mouse spleen using 87495-1-RR (same clone as 87495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87495-1-RR (Integrin Beta 1/CD29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87495-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87495-1-RR (same clone as 87495-1-PBS)

    IHC staining of mouse spleen using 87495-1-RR (same clone as 87495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87495-1-RR (Integrin Beta 1/CD29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87495-1-PBS in a different storage buffer formulation.

  • Affinity of 87495-1-RR

    Affinity of 87495-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87495-1-RR against Mouse Integrin Beta 1/CD29 were performed. The affinity constant is 1.32 nM.

Mouse Integrin Beta 1/CD29 Recombinant monoclonal antibody, PBS Only

Integrin Beta 1/CD29 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87495-1-PBS

产品说明书

CloneNo. 252764F11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CD29, Fibronectin receptor subunit beta, Integrin beta-1, Itgb1, VLA-4 subunit beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

87495-1-PBS targets Integrin Beta 1/CD29 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg10460

Product name: Recombinant mouse ITB1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011455 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin beta 1 (fibronectin receptor beta)
别名 CD29, Fibronectin receptor subunit beta, Integrin beta-1, Itgb1, VLA-4 subunit beta
观测分子量 130-140 kDa
基因名称 Itgb1
Gene ID (NCBI) 16412
RRIDAB_3745580
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09055
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrin beta-1 (ITGB1), also named as CD29, is a single-chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSCs from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, with weaker expression on granulocytes, and is not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

||
New chat

Able™

正在加载，请稍候...