验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87495-1-RR targets Integrin Beta 1/CD29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg10460 Product name: Recombinant mouse ITB1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Sequence: \ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | integrin beta 1 (fibronectin receptor beta) |
| 别名 | CD29, Fibronectin receptor subunit beta, Integrin beta-1, Itgb1, VLA-4 subunit beta |
| 观测分子量 | 130-140 kDa |
| 基因名称 | Itgb1 |
| Gene ID (NCBI) | 16412 |
| RRID | AB_3745580 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09055 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrin beta-1 (ITGB1), also named as CD29, is a single-chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSCs from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, with weaker expression on granulocytes, and is not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin Beta 1/CD29 antibody 87495-1-RR | Download protocol |
| IHC protocol for Integrin Beta 1/CD29 antibody 87495-1-RR | Download protocol |
| WB protocol for Integrin Beta 1/CD29 antibody 87495-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
