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验证数据展示

  • WB analysis of HuH-7 using 84643-1-RR

    WB analysis of HuH-7 using 84643-1-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 84643-1-RR (IGF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 84643-1-RR

    IHC staining of human ovary cancer using 84643-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 84643-1-RR

    IHC staining of human ovary cancer using 84643-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84643-1-RR (IGF2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84643-1-RR

    Affinity and Kinetic Characterization of 84643-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84643-1-RR against Human IGF2 were performed. The affinity constant is 0.159 nM.

IGF2 Recombinant monoclonal antibody

IGF2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84643-1-RR

产品说明书

CloneNo. 241746D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHuH-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84643-1-RR targets IGF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 2 (somatomedin A)
别名 241746D3, IGF II, IGF-II, Insulin-like growth factor II Ala-25 Del, PP1446
观测分子量20 kDa
基因名称 IGF2
Gene ID (NCBI) 3481
RRIDAB_3672102
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP01344
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor 2 (IGF2) is a crucial protein that plays a significant role in regulating growth, particularly during normal fetal development, and is also often dysregulated during tumorigenesis. IGF2 is one of the main ligands of the type 1 IGF receptor (IGF-1R), a tyrosine kinase receptor that transmits signals upon binding with IGF-II. IGF2 is predominantly secreted via the placenta during pregnancy and, after birth, primarily by hepatocytes, with its secretion being independent of growth hormone (GH) secretion, unlike IGF-I.

实验方案

Product Specific Protocols
WB protocol for IGF2 antibody 84643-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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