• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

IFIH1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP02938-1

Synonyms

MDA5, CADM-140 autoantigen, Clinically amyopathic dermatomyositis autoantigen 140 kDa, EC:3.6.4.13, Helicard

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02938-1, Human IFIH1 Recombinant Matched Antibody Pair - PBS only. 87135-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16467. 87135-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP02938-1

    Sandwich ELISA standard curve of MP02938-1, Human IFIH1 Recombinant Matched Antibody Pair - PBS only. 87135-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag16467. 87135-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • The mean IFIH1 concentration was determined to be 691.0 pg/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 751.3 pg/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 940.8 pg/mL in Raji cell extract based on a 1.2 mg/mL extract load and 672.1 pg/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02938-1

    The mean IFIH1 concentration was determined to be 691.0 pg/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 751.3 pg/mL in Jurkat cell extract based on a 1.2 mg/mL extract load, 940.8 pg/mL in Raji cell extract based on a 1.2 mg/mL extract load and 672.1 pg/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP02938-1 targets IFIH1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: IFIH1 Recombinant monoclonal antibody, PBS Only (Capture) 87135-3-PBS (252265C4). 100 μg. Concentration 1 mg/ml.

Detection antibody: IFIH1 Recombinant monoclonal antibody, PBS Only (Detector) 87135-2-PBS (252265G5). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Ag16467

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87135-3-PBS
Tested Reactivity human
Immunogen IFIH1 fusion protein Ag16467
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms
GenBank accession numberBC111750
Gene symbol IFIH1
UniprotQ9BYX4
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87135-2-PBS
Tested Reactivity human
Immunogen IFIH1 fusion protein Ag16467
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms
GenBank accession numberBC111750
Gene symbol IFIH1
UniprotQ9BYX4
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
||
New chat Able
正在加载，请稍候...