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验证数据展示

  • WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

    WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFI16 antibody included
  • IF Staining of HeLa using 67790-1-Ig (same clone as 67790-1-PBS)

    IF Staining of HeLa using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (67790-1-Ig, Clone: 1C10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

IFI16 Monoclonal antibody, PBS Only

IFI16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67790-1-PBS

产品说明书

CloneNo. 1C10D7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

Ifi 16, IFI16, IFNGIP1, PYHIN2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.

产品信息

67790-1-PBS targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag30314

Product name: Recombinant human IFI16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 580-729 aa of BC017059

Sequence: VCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGVFEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKVIKTRKNKKDILNPDSSMETSPDFFF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon, gamma-inducible protein 16
别名 Ifi 16, IFI16, IFNGIP1, PYHIN2
计算分子量 729 aa, 82 kDa
观测分子量85-95 kDa
GenBank蛋白编号BC017059
基因名称 IFI16
Gene ID (NCBI) 3428
RRIDAB_2918554
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16666
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

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