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Recombinant Human VEGF-B protein (hFc Tag) (HPLC verified)

种属

Human

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

hFc Tag

生物活性

未测试

Cat no : Eg0123

Print datasheet

Synonyms

VEGFB, VEGF B, VEGF related factor, VEGF-related factor, VRF

验证数据展示

  • Purity of Recombinant Human VEGF-B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human VEGF-B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • The purity of Human VEGF-B was greater than 90% as determined by SEC-HPLC.

    The purity of Human VEGF-B was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human VEGF-B protein Pro22-Ala207 (Accession# P49765-1) with a human IgG1 Fc tag at the N-terminus.
基因ID 7423
蛋白编号 P49765-1
预测分子量 45.4 kDa
SDS-PAGE 40 kDa and 50-65 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Vascular endothelial growth factor B (VEGFB) is a member of the PDGF/VEGF growth factor family. It is produced as a secreted homodimer. VEGF-B binds to VEGF receptor-1 (Flt-1) and regulates plasminogen activator activity in endothelial cells. It also binds to Neuropilin-1 (NRP1). There are two splice isoforms, VEGF-B (167) and VEGF-B (186), which have identical NH(2)-terminal cystine knot growth factor domains but differ in their COOH-terminal domains. VEGF-B (167) has a heparin-binding domain so can bind to cell-surface heparan sulphate proteoglycans upon secretion, while VEGF-B (186) does not contain the heparin-binding domain.

参考文献:

1. B Olofsson, et al. (1998) Proc Natl Acad Sci U S A. 95(20):11709-14. 
2. T Makinen, et al. (1999) J Biol Chem. 274(30):21217-22. 
3. Xuri Li. (2010) Cell Res. 20(7):741-4.
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